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KMID : 0545120040140040844
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 4 p.844 ~ p.851
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Enhancement of Erythropoietin Production from Chinese Hamster Ovary (CHO) Cells by Introduction of the Urea Cycle Enzymes, Carbamoyl Phosphate Synthetase 1 and Ornithine Transcarbamylase
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Kim NY
Lee YJ/Kim HJ/Choi JH/Kim JK/Chang KH/Kim JH/Kim HJ
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Abstract
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Efficient mammalian erythropoietin (EPO)-expression systems are required for therapeutic applications. The accumulation of ammonia is a major problem in the production of recombinant proteins in cultured animal cells. To counter this problem we introduced the first two genes of the urea cycle carbamoyl phosphate synthetase (CPSI) and ornithine transcarbamylase (OTC) into IBE Chinese Hamster Ovary (CHO) cells by stable transfection. The resulting cell line CO5 had a higher growth rate and accumulated less ammonia per cell than the parental cell line IBE. In addition it produced 2 times more EPO than the parent and the purified EPO contained a higher proportion of acidic isoforms with approximately 15% more sialic acid.
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KEYWORD
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